Multiscale modeling to understand robustness of stem cell maintenance

演題

Multiscale modeling to understand robustness of stem cell maintenance

講演者

Dr. Venu Reddy Gonehal (UC Riverside)

使用言語

English

日時

2026年9月9日(水曜日) 14：00～15：00

場所

L13 meeting room

内容

The mechanisms regulating the robustness of multicellular development are often multilayered, with underlying processes occurring at different spatio-temporal scales. Pluripotent stem cells are stably maintained in shoot apical meristems (SAMs) despite periodic differentiation of stem cell progeny into all above-ground organs and variable environmental conditions. In Arabidopsis thaliana, the stem cell-promoting homeodomain transcription factor (TF)-WUSCHEL (WUS) diffuses between cells through microscopic pores, the plasmodesmata (PD), to form a concentration gradient critical for stem cell homeostasis. WUS regulates itself by transcribing CLAVATA3 (CLV3), which encodes a secreted peptide that activates receptor kinase signaling, through a novel concentration-dependent activation-repression switching mechanism. My talk will focus on integrating multiple mechanisms that contribute to robustness. They include i) Analysis of the determinants of cooperative interaction among cis-elements within the 3 cis-regulatory modules (CRMs) and interaction among different CRMs in stable CLV3 regulation, explored through stochastic modeling. ii) The action of CLV3 signaling and plant hormones in regulating the WUS concentration gradient at multiple levels: its transcription, subcellular partitioning, and diffusion between cells explored by employing deterministic modeling. iii) Development of a hybrid model coupling both the stochastic and deterministic models to study simultaneous control of processes regulating robustness of the CLV-WUS feedback system in stem cell homeostasis. Specifically, I will present new insights that have obtained into the architecture of CRMs, CLV3 signal-dependent shadowing of cis-elements in CLV3 regulation, and the simultaneous negative and positive regulation of CLV3 signaling on WUS transcription and WUS protein regulation, respectively, in the robust maintenance of the WUS protein gradient.

問合せ先

Plant Stem Cell Regulation and Floral Patterning
Ito Toshiro (itot@bs.naist.jp)