Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation

演題

Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation

講演者

勝野　弘子　博士（Dr. Hiroko Katsuno-Kambe）
（Institute for Molecular Bioscience, The University of Queensland in Australia）

使用言語

English

日時

2021年12月9日(木曜日) 13：30～14：30

場所

Large seminar room

内容

Epithelial networks are commonly generated by processes where multicellular aggregates elongate and branch. Here we focus on understanding cellular mechanisms for elongation, using an organotypic culture system as a model of mammary epithelial anlage. Isotropic cell aggregates broke symmetry and slowly elongated when transplanted into collagen 1 gels. The elongating regions of aggregates displayed enhanced cell proliferation that was necessary for elongation to occur. Strikingly, this loco-regional increase in cell proliferation occurred where collagen 1 fibrils reorganized into bundles which were polarized with the elongating aggregates. Applying external stretch as a cell-independent way to reorganize the ECM, we found that collagen polarization stimulated regional cell proliferation to precipitate symmetry-breaking and elongation. This required b1-integrin and ERK signaling. We propose that collagen polarization supports epithelial anlagen elongation by stimulating loco-regional cell proliferation. This could provide a long-lasting structural memory of the initial axis that is generated when anlage break symmetry.

【Reference】
Katsuno-Kambe, H., Teo, J. L., Ju, R. J., Hudson J., Stehbens, S. J., Yap, S. A. (2021) Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation. eLife, 10:e67915.

問合せ先

Systems Neurobiology and Medicine
Naoyuki Inagaki (ninagaki@bs.naist.jp)